|
Cell Proliferation Assay
KK Biomed Corporation PCR Reagents Pharmaceutical Out Sourcing Products and Kits Products Services ReadyWells Cell Proliferation Genetic Drug Carrier HPLC / Drug Analysis HotBeads Cyclodextrin Polymers Taq Polymerase Free $5 Gift Card With ReadyWell Purchase!
|
|
|
MDA Kit For Cell Proliferation Assays
Description: The mitochondrial dehydrogenase assay (MDA) kit is a spectrophotometric assay of cell growth as a function of mitochondrial activity in living cells. The MDA assay measures the viability of living cells as a function of mitochondrial dehydrogenase activity with 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) substrate. Mitochondrial dehydrogenases of viable cells cleave the tetrazolium ring, yielding purple MTT formazan crystals which are insoluble in aqueous solution. Greater numbers of viable cells correlate with more production of MTT formazan and the formazan product is assayed by absorbance at 570nm. The MDA assay is a proven method for determination of cell viability and proliferation in mammalian cells (1, 2). Cell viability is often reported as % viability relative to control. The % cell viability can be calculated as [1-Sample ABS/Control ABS]*100.
Kit Components: 5X Assay Reagent 1X Formazan Diluent Instruction Sheet
Applications: Determine cell proliferation, cell viability, and cell death by absorbance. Quantify total viable cells in a population.
Features: Proprietary formulation gives higher signal, lower background, than other MTT based assays on the market. Rapidly quantify living cells in as little as 2 hours. Proven chemistry. Validated in many mammalian cells, including COS7, 3T3, MCF7 and BHK21.
MDA Kit produces higher signal than other MTT based assays.
Comparison of MDA Kit with competitor assay in four cell lines.
Determine drug toxicity at the micromolar level. 
References: 1) Benimetskaya L., Guzzo-Pernell N., Liu S.T., Lai J.C.H., Miller P., and Stein C. A. (2002) Protamine-fragment peptides fused to an SV40 nuclear localization signal deliver oligonucleotides that produce antisense effects in prostate and bladder carcinoma cells. Bioconjugate Chem. 13, 177-187. 2) Chi NK, Wallis A.E., Lee C.H., Menezes D.L.D, Sartor J., Dragowska W.H., and Mayer L.D. (2000) Effects of Bcl-2 modulation with G3139 antisense oligonucleotide on human breast cancer cells are independent of inherent Bcl-2 protein expression. Breast Cancer Research and Treatment 63, 199-212. FOR LABORATORY RESEARCH ONLY, NOT FOR DIAGNOSTIC USE. MDA Kit PricingPurchase Orders Welcome by E-Mail or Call: (800) 666-9677
Prices are in US Dollars F.O.B. KK Biomed with the cost of taxes and shipping added to invoice. KK Biomed exclusively warrants that its products perform as described, subject to replacement or refund limited to the purchase price. KK Biomed expressly disclaims warranties of fitness for some other purpose. KK Biomed does not warrant that the use of its products will not infringe the claims of any patents. E-mail for Information, Sales and Customer Support:
info@kkbiomed.com |
